Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

• NCJ Number: 302740
• Journal: Forensic Science International: Genetics Volume: 7 Issue: 1 Dated: 2019 Pages: 485-487
• Author(s): Bruce McCord; Quentin Gauthier; Hussain Alghanim; Joana Antunes; Nicole Fernando Tejero; George Duncan; Kuppareddi Balamurugan
• Date Published: 2019
• Length: 3 pages
• Grant Number(s): 2017-NE-BX-0001
• Agencies: NIJ-Sponsored
• Award: Implementation of epigenetics into forensic science: Development of a PCR based multiplex for the simultaneous analysis of age and body fluid identification
• Publication Type: Research (Applied/Empirical)

Annotation

In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. 

Abstract

The first application involves the development of a multiplex amplification capable of simultaneous analysis of four different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. The authors have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status. (publisher abstract modified)